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Evaluating Effect of Nesfatin-1 on cardiac function in heart failure induced by Isoproterenol in male Wistar rat.
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Mahdi Saberi Pirouz   , Kamran Rakhshan , Yaser Azizi |
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Abstract: (269 Views) |
Introduction: Heart failure following ischemic injuries, by reducing cardiac output, reduces blood supply to other organs and induces damage to them. Nesfatin-1, as an adipocrine peptide, with antioxidant and anti-inflammatory effects in brain tissue might also reduce cardiac injuries in heart failure.
Methods and Materials: 36 male Wistar rats were divided into 3 groups of control (CTR), nesfatin-1 (Nesf-1), heart failure (HF) and heart failure+nesfatin-1 (HF+Nesf-1). Heart failure was induced by subcutaneous injection of 130 mg/kg isoproterenol in four consecutive days. 28 days after the last injection, Nesfatin-1 (10 mg/kg, ip) was injected into the subjects for 5 days. Heart weight to body weight ratio, Ejection Fraction (EF) and Fractional shortening (FS) as heart functional indices (by echocardiography), cardiac fibrosis (Masson trichrome staining), histological changes (H&E staining) and serum cTnI (ELISA) were evaluated.
Results: Cardiac function in HF group showed a significant decrease compared to CTL group (P<0.001). Treatment with nesfatin-1 increased these indices compared to HF group (P<0.05). The serum level of cTnI in HF group was significantly higher than the control group (P<0.01), and this level was evidently decreased in HF+Nesf-1 group (P<0.05). The amount of cardiac fibrotic tissue in HF rats was clearly higher than healthy rats (P<0.001), that in HF+Nesf-1, the accumulation of collagen fibers decreased significantly compared to the HF group (P<0.05). Destructive tissue changes in HF+Nesf-1 rats were clearly reduced compared to the heart failure group.
Conclusion: It seems that Nesfatin-1 has cardioprotective effects by improving tissue destructive changes, reducing cardiac fibrosis and increasing left ventricular contractility and can reduce the damage caused by isoproterenol.
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| Keywords: Heart failure, Nesfatin-1, Cardiac function, Isoproterenol |
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Full-Text [PDF 270 kb]
(35 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2023/09/16 | Accepted: 2023/08/23 | Published: 2023/08/23
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