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Cancer cell detection using electrochemical nanobiosensor based on graphene / gold nanoparticle
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Meisam Omidi   , Amir Yadegari , Hakimeh Zali , Mohadese Hashemi , Hadi Hasanzadeh |
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Abstract: (6588 Views) |
| Introduction: Nowadays, early cancer detection and effective treatment is crucial for improved prognosis and cancer management. In particular, the accurate qualitative detection of cancer cells represents a critical step in cancer diagnosis. The aim of this study was to examine Cancer cell detection using electrochemical nanobiosensor based on graphene / gold nanoparticle.
Materials and Methods: Modified graphene oxide/gold nanoparticle electrodes were employed to increase the sensitivity of human breast cancer MCF-7 cells detection, using CD44 biomarker. Frist the electrodes were modified with graphene, then gold nanoparticles were sediment on graphene-modified electrode. Then CD44 monoclonal antibody conjugated on the surface of gold nanoparticles, on graphene-modified electrode. Finally, the performance of the fabricated biosensors were investigated by using a common reference electrode composed of silver-silver chloride and a common platinum counter electrode at different antigen concentrations with the buffer and serum.
Results: The proposed electrochemical cytosensor delivered a high sensitivity with the average of 1.12 μA / cells ml-1, and a low detection limit of 6 cells.
Conclusion: These results indicate that the cytosensor has great potential in diagnosis of cancers |
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| Keywords: Electrochemical nanobiosensor, Human breast cancer MCF-7 cells, CD44 Monoclonal antibody |
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Full-Text [PDF 812 kb]
(1809 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2015/11/21 | Accepted: 2016/06/5 | Published: 2016/09/21
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