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Evaluating two indirect hemagglutination methods in diagnosis of listeriosis in comparison with indirect immunoflourecens
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B. صدیقی مقدم   |
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Abstract: (14854 Views) |
| Introduction: Ba ct e rimi a res u lte d fro m Lis t eri a mono cyt ogen e s lea ds to va rIOUS
complications including abortion . While cult uring the specimen is a crocial me thod in diagn osing
of liste ri osi s, it has low se ns it ivity (because o f the few number of bacteria in specimens) and
takes long time. Serological methods are used routinely in many diagnostic laboratories because
of improved time and sensitivity.
Materials and methods: IHA (indirect hemagglutination) was performed using fract ion 1,
obtai ne d from ge l chroma tograp hy of sonica te d liste r ia monocytoge ne s cr ude antige ns. IHA
th rou gh pot e l's me th od was ca rried on. Se ns it ivity and specific ity of th ese methods compa re d
with current IFA (immunotlourecens assay) in diagnos ing the Listeriosis. The titers of antibody
against Listeri a mon ocytoge nes were de te rmine d in se rums of 207 women having at le ast o ne
abortion. Among this group serums of 96 women by IHA with soluble antigens in fra ction I and
82 women by IHA with soluble antigen in potel's method were tes ted .
Results: In IFA (sensitivity 70% and spec ificity 50%),83.5% had valuable titer s of antibody.
Niether crude antige n n or t hr ee fr ac tio ns we re suitab le for IHA, bu t al182 serums (pote l's
method) showing 1/4 to 1/256 tite rs (se nsit ivity 36% and spec ificity 96%) .
Conclusion: IFA is the most cu rrent method b ut, because o f h igh sensit ivity a nd low
specific ity a more confident meth od is required. This study shows antigenic fraction s of listeria
monocytoge nes are no t suitab le for IHA, while cha ng ing the co nd itio n of cu ltu re in po tel's
method leads to the applicable antigen for IHA. Lack of correlation betwee n IFA and potel's
IHA indicate that the nature of antigens arc diffe rent in bot h me thods. Thus it is recommended
to use IFA for screening and simultaneously potel's IHA (because of high specificity). |
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| Keywords: Indirect immunotlourecens (IFA), Indirect hemagglutinati on (IHA), Liste riosis |
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Full-Text [PDF 2490 kb]
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Type of Study: Research |
Subject:
General
Received: 2008/12/23 | Published: 2002/08/15
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