|
Visual deprivation during critical period of brain development inhibits expression of glutamic acid decarboxylase enzyme in rat hippocampus
|
Sayyed Alireza Talaei   , Abolfazl Azami , Mahmoud Salami |
|
|
|
Abstract: (4676 Views) |
| Introduction: Visual deprivation during critical
period of brain development inhibits the GABAergic system growth and completion
in visual cortex. Because parts of visual signals are transferred to
hippocampus through the visual cortex, the aim of this experiment was to study
the effects of visual deprivation during critical period of brain development
on expression of Glutamic Acid Decarboxylase enzyme in rats’ hippocampus. Materials and methods: This study was carried on 2 groups
(n=36) of male rats kept in standard 12 hour light/dark cycle (Light reared, LR)
or in complete darkness (Dark reared, DR). Each group, in turn, was divided
into 3 aged-based subgroups of 2, 4 and 6 weeks old rats. Using RT-PCR and
Western Blot techniques, expression of both isoforms of Glutamic Acid
Decarboxylase enzyme were investigated in hippocampus. Results: Relative expression of mRNA and protein
of GAD65 and GAD67 enzymes increased in the LR animals, time dependently.
However, although the expression of both enzymes increased in the DR animals by
aging, but, the expression was much lower in compare to the LR rats. Conclusion: Visual deprivation
during critical period of brain development inhibits the expression of GAD65
and GAD67 enzymes in rat hippocampus and delays GABAergic system development |
|
| Keywords: Visual Perception, Sensory Deprivation, Critical Period, Hippocampus, Glutamate Decarboxylase, Rats |
|
|
Full-Text [PDF 949 kb]
(976 Downloads)
|
Type of Study: Research |
Subject:
General
Received: 2014/07/9 | Accepted: 2015/01/28 | Published: 2015/06/10
|
|
|
|
|
|
|
| Send email to the article author |
|
|
|
| Add your comments about this article |
|
|
|
