اکثر مکاتبات کومش از طریق ایمیل سایت می باشد. لطفا Spam ایمیل خود را نیز چک نمایید.
   [Home ] [Archive]   [ فارسی ]  
:: ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
Subscription::
Contact us::
Site Facilities::
Webmail::
Editorial Board::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
:: Volume 24, Issue 5 (Sep and Oct 2022) ::
Koomesh 2022, 24(5): 684-690 Back to browse issues page
Molecular investigation of Campylobacter spp. and salmonella infection in children with community acquired diarrhea
Haniyeh Ahadi , Bahareh Attaran , Roxana Mansour Ghanaie , Leila Ganji , Fatemeh Fallah , Abdollah Karimi , Iraj Sedighi , Marjan Tariverdi , Alireza Nateghian , Negin Nahan-Moghadam , Masuod Alebouyeh
Abstract:   (637 Views)
Introduction: Acute gastroenteritis is a typical disorder that accounts for 8-12% of pediatric outpatient visits. Campylobacter and Salmonella infections account for about 8.4% and 11% of global diarrhea cases. Due to the importance of these bacteria in pediatric diseases, the aim of this study was to determine the infectious rate of Salmonella and Campylobacter species and also the frequency of the gene encoding Cytholethal distending toxin in children with community-acquired diarrhea.
Materials and Methods: Stool samples of children under 5 years of age with diarrhea were collected. The samples were related to children referred to hospitals in Hamadan, Ardabil, Bandar Abbas and two hospitals in Tehran. DNA was extracted from the samples using a DNA extraction kit from stool. The presence of Campylobacter in the studied samples was detected by polymerase chain reaction using specific primers. A control stool sample was spiked with 10-fold dilution of C. jejuni suspension for LOD (detection limit determination) measurement.
Results: In this study, PCR results showed a LOD of 100 CFU per gram in the spiked feces sample. Accordingly, out of 144 fecal samples of children with acute diarrhea, one case was positive for Campylobacter jejuni; this sample was also positive for the presence of cdtB gene. Presence of Salmonella was confirmed in two samples of the patients (1.4%).
Conclusion: Low prevalence of Campylobacter and Salmonella was detected in symptomatic children under 5 years of age during the Covid-19 pandemic. Examination of these samples for viruses and other microbial agents can clarify the etiology of diarrhea in children referred to the hospitals.
Keywords: Diarrhea, Children, Salmonella, Campylobacter, Cytolethal distending toxin
Full-Text [PDF 1107 kb]   (426 Downloads)    
Type of Study: Research | Subject: General
Received: 2021/12/29 | Accepted: 2022/04/17 | Published: 2022/09/27
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA

Ethics code: IR.SBMU.RICH.REC.1400.020


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ahadi H, Attaran B, Mansour Ghanaie R, Ganji L, Fallah F, Karimi A, et al . Molecular investigation of Campylobacter spp. and salmonella infection in children with community acquired diarrhea. Koomesh 2022; 24 (5) :684-690
URL: http://koomeshjournal.semums.ac.ir/article-1-7446-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 24, Issue 5 (Sep and Oct 2022) Back to browse issues page
کومش Koomesh
Persian site map - English site map - Created in 0.06 seconds with 38 queries by YEKTAWEB 4645