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:: Volume 23, Issue 1 (زمستان 1399) ::
Koomesh 1399, 23(1): 124-130 Back to browse issues page
Effect of serum starvation stress on the mouse spleen mononuclear cells mixed culture: Introducing a new immunomodulatory method
Gilda Parsamanesh , Maryam Mehri , Mostafa Sheikhzadeh , Seyyed Hossein Mousavie Anijdan , Amrollah Mostafazadeh
Abstract:   (2527 Views)
Introduction: Bone marrow and immune cell transplants are a common method of treating some blood diseases around the world. However, due to the malfunction of the immune system, its use is not always effective. In this study, we evaluated the potential of culture stress in serum-free medium in regulating the immune system. Materials and Methods: Spleen immune cells were isolated from Balb/C and C57bl/6 mice using the Ficol gradient method. C57bl/6 mice splenocytes were cultured in serum starved and non-starved conditions at indicated time points. Cell viability was determined by MTT assay. Then the allogenicity of these cells was determined by mixed lymphocytic culture (in vitro model of bone marrow transplantation) with Balb/C mouse splenocytes and cell proliferation was then examined using MTT method and necessary photographs were prepared too. Results: The viability rate of starved immune cells after 72 h was 82.1% of the control group. After 24h, the allogenicity of these cells decreased significantly compared to the control cells (P
Keywords: Transplantation, Cell culture Technique, Mouse, Immunoregulation
Full-Text [PDF 727 kb]   (633 Downloads)    
Type of Study: Research | Subject: General
Received: 2019/12/26 | Accepted: 2020/06/24 | Published: 2021/01/23
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Ethics code: MUBABOL.REC.1394.82



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Parsamanesh G, Mehri M, sheikhzadeh M, Mousavie Anijdan S H, Mostafazadeh A. Effect of serum starvation stress on the mouse spleen mononuclear cells mixed culture: Introducing a new immunomodulatory method. Koomesh 1399; 23 (1) :124-130
URL: http://koomeshjournal.semums.ac.ir/article-1-6096-en.html


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Volume 23, Issue 1 (زمستان 1399) Back to browse issues page
کومش Koomesh
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