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The metabonomic changes of leishmania major, s promastigotes (fredlin strain) after in vitro artemisinin treatment at stationary phase
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Masoud Beigi boroujeni   , Mohammad Arjmand , Ghader Khalili , Ziba Akbari , A. li Najafi , Nasim Beigi boroujeni , Afsaneh Shafiei , Reza Haji hosseini |
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Abstract: (17613 Views) |
| Introduction: Based on WHO classifications, leishmaniasis
enumerates as one of the six important diseases in the tropics. It is also
common in the Middle East and the Mediterranean countries. Control of the disease
is important due to increased clinical cases in immune-system deficiency
patients. With regard to the increasing need to new drugs with lesser side
effects for the control and treatment of the disease, we surveyed the in
vitro effects of artemisinin on the promastigotes of leishmania major,
s metabolites in Pasteur institute of Iran. Materials and Methods: After IC50 determination, the effects of
different concentrations of artemisinin drug were assessed on the metabolites
of leishmania in two groups the control group (without drug reception
in culture media) and the case group (with drug reception in culture media), by
using NMR technique. Results: The results of this study indicate that in
the metabolic changes in promastigotes of leishmania major (fredlin
strain) at stationary phase, 5 metabolites of the 41 ones in the galactose
metabolic cycle have shown significant changes. Therefore, galactose metabolism
has changed the most, while sphingolipid, valine, leucine and isoleucine
biosynthesis follow respectively. Conclusion: Artemisinin resulted in metabolic changes in
biochemical pathways including galactose, sphingolipids and also valine,
lucine, isoleucine that culminated in cessation of leishmania major’s
(fredlin strain) activity. Spectra derived from H NMR showed significant
difference (p |
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| Keywords: Artemisinin, Leishmania, parasite, Fredlin, Metabonomics |
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Full-Text [PDF 673 kb]
(16706 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2013/12/25 | Accepted: 2014/06/8 | Published: 2014/09/13
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