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:: Volume 1, Issue 1 (11-1999) ::
koomesh 1999, 1(1): 29-35 Back to browse issues page
HPLC measurment of phenylalanine in plasma, with application to patients with phenylketonuria
A. R. Bandegi * , M. Doosti
Abstract:   (11651 Views)
Introduction. Diagnosis and treatment of phenylketonuria (PKU) requires measurment of phenylalanine (Phe) in serum or plasma. The aim of this study was to use a simple and rapid method for measurment of Phe. Materilas and Methods. Phe is determined by isocratic high performance liquid chromatography (HPLC) method, with UV-VIS detection in 210 nm , octadecyl-silane (ODS) column and the mobile phase of aceton itrillwater. For this measurment 10 microliter plasma was used and complet analysis was done at less than 12 min from injection. Results. Coeifficients of variation (% CV) for human plasma sample (Phe, 60-1200 mmol/L) ranged between 2.8% and 3.3%. The method has wide linear range (10-3000 mmol/L). Conclusion. In this method preparation of sample is simple and rapid. This method has an high efficiency for measurment of Phe concentration in the normal and PKU subjects.
Keywords: Phenylketonuria; Phenylalanine; Measurment; Plasma; High Performance Liquid Chromatography
Full-Text [PDF 868 kb]   (1697 Downloads)    
Type of Study: Research | Subject: General
Received: 2009/02/2
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Bandegi A R, Doosti M. HPLC measurment of phenylalanine in plasma, with application to patients with phenylketonuria. koomesh. 1999; 1 (1) :29-35
URL: http://koomeshjournal.semums.ac.ir/article-1-385-en.html


Volume 1, Issue 1 (11-1999) Back to browse issues page
کومش Koomesh
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