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:: Volume 21, Issue 1 (winter 2019) ::
koomesh 2019, 21(1): 171-180 Back to browse issues page
In vitro anti-bacterial effect of nano-polyamidoamine-G5 dendrimer
Soudabeh Alizadeh Matboo , Shahram Nazari * , Leila Mohammadi , Susan Bagheri , Fakhraddin Akbari Dourbash , Ahmadreza Yari , Seyyed Ahmad Mokhtari , Ali Niapour , Seyyed Mohsen Mohseni
Abstract:   (65 Views)
Introduction: Progress in nanotechnology in the past decayed has created various opportunities for evaluation of biological effects such as anti-bacterial effects of nanoparticles.  This study was aimed to examine synthesis and the antibacterial effect of Nano-Polyamidoamine-G5 (NPAMAM-G5) dendrimer on Klebsiella Pneumoniae, Pseudomonas Aeruginosa, Shigella Dysenteriae and Bacillus Subtilis.
Materials and Methods:  NPAMAM-G5 dendrimers was synthesized by Tomalia’s divergent growth approach. The antibacterial effects of NPAMAM-G5 dendrimer were studied by disc diffusion and micro-dilution method. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against gram-positive and gram-negative bacteria were determined according to Clinical and Laboratory Standards Institute (CLSI) guideline. Transmission electron microscopy (TEM) was used to analyze morphology and size of NPAMAM-G5.
Results: Zone of inhibition in concentration 25μg/ml of NPAMAM-G5 dendrimers for Klebsiella Pneumoniae, Pseudomonas Aeruginosa, Shigella Dysenteriae and Bacillus Subtilis were 27, 13, 30 and 18 mm, respectively. There was a significant difference regarding the zone of inhibition between gram-negative and gram-positive bacteria (p<0.05). Remarkably, the MIC for Klebsiella Pneumoniae, Pseudomonas Aeruginosa and Shigella Dysenteriae was 2.5μg/ml and for Bacillus Subtilis was 25μg/ml. The MBC for Shigella Dysenteriae and Pseudomonas aeruginosa were 50 and 200 μg/ml, respectively and for Klebsiella Oxytoca and Bacillus Subtilis was100 μg/ml. It was found that NPAMAM-G5 particles had a spherical shape with a mean diameter size of 10 nm.
Conclusion: According to the results, the NPAMAM-G5 dendrimer with end amine groups displayed a positive effect on the removal of standard strains of gram-positive and gram-negative bacteria.
Keywords: Gram Positive Bacteria, Gram Negative Bacteria, Dendrimers, Polyamines, Microbial Sensitivity Tests
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Type of Study: Research | Subject: General
Received: 2016/09/10 | Accepted: 2018/08/1 | Published: 2019/01/7
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Alizadeh Matboo S, Nazari S, Mohammadi L, Bagheri S, Akbari Dourbash F, Yari A, et al . In vitro anti-bacterial effect of nano-polyamidoamine-G5 dendrimer . koomesh. 2019; 21 (1) :171-180
URL: http://koomeshjournal.semums.ac.ir/article-1-3548-en.html

Volume 21, Issue 1 (winter 2019) Back to browse issues page
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