[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 19, Number 3 ( Summer 2017, 19 (3). 505-711 2017) ::
koomesh 2017, 19(3): 611-618 Back to browse issues page
Inhibitory effect of luteolin on MKN-45 tumorg gastric cells: The role of nucleoside-diphosphate kinases, cofilin and actin proteins
Zahra Tahmasebi, Maziar Mohammad Akhavan *, Sepideh Torabi, Naser Abbasi, Elham Sarhadi, NAsim Hatam nejadian
Abstract:   (178 Views)

Introduction: Luteolin is an important plant extract. This shows anti-inflammatory, inhibition of cell growth, induction of apoptosis and inhibition of metastasis. Gastric cancer is one of the most common cancers in the world. Also metastasis is widespread in this kind of cancer and late diagnosis is a cause of patient's death.  Cofilin, actin and nucleoside-diphosphate kinases (NDPK) are important in cell death and metastasis. In this study the effect of luteolin on MKN-45 gastric carcinoma cells was evaluated.

Materials and Methods: At first the cells were cultured and then for 48 hours were cultured with the drug concentration. Then the cells where collected and their protein were extracted and analyzed using 2D electrophoresis and separated on the basis of their molecular mass and PI. After that these samples were used for determine the amino acid sequence by MOLDI-TOF.

Results: The growth rate of MKN-45 inhibited under influence of luteolin, this means increasing Apoptosis. Based on the results presented here by identification of protein spots by MOLDI-TOF mass spectrometry the expression of three protein cofilin, actin and NDPK has been decreased by luteolin.

Conclusion: It seems that the anti- tumoral effect of luteolin is expressed through three proteins cofilin, actin and NDPK in gastric cancer cells

Keywords: Luteolin, Stomach Neoplasms, Cofilin, Actins, Nucleoside-Diphosphate Kinase, Electrophoresis
Full-Text [PDF 563 kb]   (27 Downloads)    
Type of Study: Research | Subject: General
Received: 2016/07/26 | Accepted: 2017/04/8 | Published: 2017/06/21
Send email to the article author

Add your comments about this article
Your username or email:

Write the security code in the box >



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Tahmasebi Z, Akhavan M M, Torabi S, Abbasi N, Sarhadi E, Hatam nejadian N. Inhibitory effect of luteolin on MKN-45 tumorg gastric cells: The role of nucleoside-diphosphate kinases, cofilin and actin proteins. koomesh. 2017; 19 (3) :611-618
URL: http://koomeshjournal.semums.ac.ir/article-1-3453-en.html
Volume 19, Number 3 ( Summer 2017, 19 (3). 505-711 2017) Back to browse issues page
کومش Koomesh
Persian site map - English site map - Created in 0.083 seconds with 797 queries by yektaweb 3430