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Molecular typing of Mycobacterium tuberculosis by using double repetitive element- polymerase chain reaction method
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G. h. ایراجیان   , M. H. کیوان امینه , A. فاضلی , M. R. مسجدی , A. A. ولایتی |
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Abstract: (16161 Views) |
| Introduction . Tuherculosis is still a major wor ldwide health problem. It is responsible
for the death of 3 million people each year. E pidemio logica l stu dies with techniques
which a llow differ entia tion of stra ins within th e Mycobactrium tuberculosis (MT) are
imp ortan t to limit the disseminati on of the disease . The refore, there is a gr eat need for
an improved methods to sub type MT strains by a simp le and ra pid molecul ar
finger-printing method by usinug double repetitive eleme nt-polymerase chain reaction
(ORE-PCR) an alysis, yield s a unique, str ain -sp ecific pattern of hands.
Materials and Methods. MT was ob ta ine d from the national research institute of
Tu berculos is and lung diseases. To perform ORE-PCR, the DNA molecules were
extracted, then ampilicated with PCR. Amplication prod ucts were ana lyze d by ge l
electrophores is on 2% agarose and gen erated pa tt erns com pared with togeth er. Those
patterns that ide ntical for two or more str ains among thei r 70 MT, were co nsidered as
cluster patt erns.
Results. Fo urty-two different patt erns were ob served in the 70 isolat ed cases and
an alyzed. The ra te of diversity was 60%, which is the same (approved) as the ot he r
studies. Thirty-one (44.3%) of the specimens had unique pattern, a nd 39 (55 .7%) of
specimens had strains be longing to on e of th e 11 clusters. The patterns had 1-6 band
them a pprov ed the ot her studies. There was no cor relation found among th e sp ecial
pa tterns, resistance to ant ituberculosis agents, residan ce, migration, and Iran ian
patients. The results are th e same as what is reported in th e literature.
Conclusion. The data show th e DRE-PCR method is th e simplest a nd the fa stest
way to achieve molecular typ ing of MT in Iran. |
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| Keywords: Mycobacterium tuberculosis, PCR, ORE-PCR |
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Full-Text [PDF 1273 kb]
(2326 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2009/01/21 | Published: 2000/08/15
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