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:: Volume 19, Number 3 ( Summer 2017, 19 (3). 505-711 2017) ::
koomesh 2017, 19(3): 634-640 Back to browse issues page
Effect of berberine on cancer cell viability of chronic lymphocytic leukemia patients in in vitro
Farahnaz Ghahremanfard *, Arash Aghabeigi, Ehsan Manouchehri, Mehrnoosh Pashaei, Majid Mirmohammadkhani, Mahdieh Shokrollahi Barough, Fatemeh Pak, Mohammad Faranoush, Parviz Kokhaei
Abstract:   (183 Views)

Introduction:Chronic lymphocytic leukemia (CLL) is the most common leukemia in the Western world. CLL generally affects older people by the accumulation and proliferation of B lymphocytes with markers CD19+, CD20+, CD5+ and CD23+ specified. In this way, berberine, including natural compounds with isoquinoline alkaloids structure, that has a potential anti-cancer effect. Berberin decreased survival and increased apoptosis of cancer cell lines in in vitro. In this study, the effect of berberine on cell survival and apoptosis in PBMC patients and healthy donors in the presence or absence of berberine and also comparison between bernerine and fludarabine were investigated.

Materials and Methods:Peripheral blood mononuclear cells (PBMCs) of 12 patients and 6 healthy donors were isolated by using ficoll technique and PBMC cells seeded into the 96 well culture plate using medium RPMI1640+ AB+ serum with doses ranging from 5 to 200 μM of Berberine. The survival and apoptosis percentage of these cells was measured by MTT and Annexin V/7AAD (Flowcytometery).

Results:Our results suggest a reduction in PBMC survival in CLL patients treated with berberine with P<0.05 as compared to patients cells which not treated. Comparing PBMC survival treated with berberine to cells treated with fludarabine showed no significant change (P>0.05). Correspondingly, increasing PBMC apoptosis in patients treated with berberine was observed rather than the cells were not adjacent to berberine (P<0.05) and also compared with PBMC apoptosis treated with fludarabine which shown no significant difference (P >0.05). However, we don’t have this increasing in healthy donors.

Conclusion: Berberine leads to reduced viability and elevated levels of apoptosis in PBMCs of CLL Patients. Berberin showed no significant effects on PBMCs of healthy donor viability and apoptosis. Apoptosis induction by berberin is comparable to fludarabine as a standard treatment for CLL patients. Berberine might be a good candidate for clinical trial for CLL patients

Keywords: B-Cell Chronic Lymphocytic Leukemia, Berberine, Fludarabine, Apoptosis, Annexin 5A
Full-Text [PDF 942 kb]   (41 Downloads)    
Type of Study: Research | Subject: General
Received: 2016/05/2 | Accepted: 2017/05/27 | Published: 2017/06/21
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Ghahremanfard F, Aghabeigi A, Manouchehri E, Pashaei M, Mirmohammadkhani M, Shokrollahi Barough M, et al . Effect of berberine on cancer cell viability of chronic lymphocytic leukemia patients in in vitro. koomesh. 2017; 19 (3) :634-640
URL: http://koomeshjournal.semums.ac.ir/article-1-3329-en.html
Volume 19, Number 3 ( Summer 2017, 19 (3). 505-711 2017) Back to browse issues page
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