Antibiotic resistance patterns and plasmid profiles of 200 clinically ioslated Staphyloococcus aureus
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G. h. بهزادیان , M. انوری |
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Abstract: (12740 Views) |
Introduction. Many investigations have, so far , carried out to determine antibiotic resistance
pattern and plasmid profiles . The aim of this study was to assess coagulase positive
Staphylococcus resistance to 12 selected antibiotics, using disc diffusion and to determine
their possible origin, through plasmid deletion, using mitomycin C treatment.
Material and Methods. Bacterial strains were isolated from clinical specimens taken from
patients in Imam Khorneini (RAH) hospital and preserved at our laboratory. Being cultured
in nutrient and blood agar, they were incubated for 24 hours at 37 °C. Gram staining,
coagulase and DNAase monitol fermentation tests were done. After preparing inoculating
suspention, disc diffusion (12 antibiotics) test was performed. Susceptible and resistant
strains were also determined through their treatment with mitomycin C.
Results. The results indicated that antibi otic resistance was the most against erthrornycine
(89%) and mitomycin C significantly decreased the frequency of single, double and multiple
resistant cases.
Conclusion. These findings suggest that coagulase positive, Staphylococcus aureus is resistant, to different degree, to some selected antibiotics . Plasmids not only cause resisance to aantibiotic but also play obvious role in multiple resistance. |
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Keywords: Plasmid, Mitomycin C, Staphyloococcus aureus, Resistance, Antibiotic |
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Type of Study: Research |
Subject:
General Received: 2009/01/13 | Published: 2001/02/15
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