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:: Volume 2, Issue 2 (11-1379) ::
Koomesh 1379, 2(2): 67-71 Back to browse issues page
Antibiotic resistance patterns and plasmid profiles of 200 clinically ioslated Staphyloococcus aureus
G. h. بهزادیان , M. انوری
Abstract:   (12740 Views)
Introduction. Many investigations have, so far , carried out to determine antibiotic resistance pattern and plasmid profiles . The aim of this study was to assess coagulase positive Staphylococcus resistance to 12 selected antibiotics, using disc diffusion and to determine their possible origin, through plasmid deletion, using mitomycin C treatment. Material and Methods. Bacterial strains were isolated from clinical specimens taken from patients in Imam Khorneini (RAH) hospital and preserved at our laboratory. Being cultured in nutrient and blood agar, they were incubated for 24 hours at 37 °C. Gram staining, coagulase and DNAase monitol fermentation tests were done. After preparing inoculating suspention, disc diffusion (12 antibiotics) test was performed. Susceptible and resistant strains were also determined through their treatment with mitomycin C. Results. The results indicated that antibi otic resistance was the most against erthrornycine (89%) and mitomycin C significantly decreased the frequency of single, double and multiple resistant cases. Conclusion. These findings suggest that coagulase positive, Staphylococcus aureus is resistant, to different degree, to some selected antibiotics . Plasmids not only cause resisance to aantibiotic but also play obvious role in multiple resistance.
Keywords: Plasmid, Mitomycin C, Staphyloococcus aureus, Resistance, Antibiotic
Full-Text [PDF 818 kb]   (2074 Downloads)    
Type of Study: Research | Subject: General
Received: 2009/01/13 | Published: 2001/02/15
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بهزادیان G H, انوری M. Antibiotic resistance patterns and plasmid profiles of 200 clinically ioslated Staphyloococcus aureus. Koomesh 1379; 2 (2) :67-71
URL: http://koomeshjournal.semums.ac.ir/article-1-316-en.html


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Volume 2, Issue 2 (11-1379) Back to browse issues page
کومش Koomesh
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