اکثر مکاتبات کومش از طریق ایمیل سایت می باشد. لطفا Spam ایمیل خود را نیز چک نمایید.
   [Home ] [Archive]   [ فارسی ]  
:: ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
Subscription::
Contact us::
Site Facilities::
Webmail::
Editorial Board::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
:: Volume 18, Issue 1 (پائیز 1395: جلد 18 شماره (1) 1395) ::
Koomesh 1395, 18(1): 62-70 Back to browse issues page
Endometrial stem cells differentiation into neural cells by LY294002 small molecule
Homa Mohseni kouchesfahani , Somayeh Ebrahimi Barough , Jafar Ai , Hamideh Anbar
Abstract:   (6457 Views)
Introduction: Endometrial stem cells (EnSCs) were identified for the first time in 2004. These cells are capable of extensive self-renewal and have the potency to differentiate into chondrocyte, osteocyte, adipocyte, neuron and oligodendrocyte. PI3K/Akt signaling has been implicated in multiple cellular and organ functions, including differentiation, survival and cell death and its inhibition leads to cell differentiation. The purpose of this study was to investigate the differentiation of endometrial stem cells into neural cells by inhibition of PI3K/Akt pathway using small molecule Ly294002. Materials and Methods: Endometrial tissues were treated enzymatically and segregated cells were cultured in DMEM/F12 with 10% FBS. The flow cytometry analysis was perfomed for CD105, CD90, CD146, CD31 and CD34 at the third passage. Then the neurogenic differentiation was evaluated at the third passage, 21 days after induction with differentiation media. Immunocytochemistry and Real-time PCR were performed to investigate the expression of specific neural stem cells markers. Results: The flow cytometry analysis showed that EnSCs were positive for CD90, CD105 and CD146 and negative for CD31 and CD34. Immunocytochemistry showed that the expression of nestin, NF and Chat neuronal markers in the cells treated with small molecule Ly294002. Real-time PCR also indicated expression of NF and Chat neuronal markers at the mRNA level. Conclusion: According to the findings of this study it can be concluded that the EnSCs have neural differentiation potency in the suitable differentiation milieu. Ly294002 small molecules by inhibiting PI3K / Akt pathway possibly can prevent cell proliferation and induce cell differentiation
Keywords: Endometrium, Cell Differentiation, Stem Cells, Neural Cells, Phosphatidylinositol 3-Kinases
Full-Text [PDF 889 kb]   (1686 Downloads)    
Type of Study: Research | Subject: General
Received: 2015/12/3 | Accepted: 2016/05/15 | Published: 2016/09/18
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mohseni kouchesfahani H, Ebrahimi Barough S, Ai J, Anbar H. Endometrial stem cells differentiation into neural cells by LY294002 small molecule. Koomesh 1395; 18 (1) :62-70
URL: http://koomeshjournal.semums.ac.ir/article-1-3139-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 18, Issue 1 (پائیز 1395: جلد 18 شماره (1) 1395) Back to browse issues page
کومش Koomesh
Persian site map - English site map - Created in 0.06 seconds with 38 queries by YEKTAWEB 4645