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Induction of respiratory burst in human monocytes after treating with platelet-derived microparticles
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ُSamaneh Koushki   , Fatemeh Yari , Mojgan Shaiegan |
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Abstract: (4400 Views) |
| Introduction: Activated platelets in platelet concentrate, release membranous vesicles that called platelet microparticles and can be created spontaneously during the storage of platelets. Studies have shown that platelet microparticles can form aggregates with monocytes so we intended to survey the ability of these microvesicles to activate monocytes.
Materials and Methods: Platelet concentrate bags (PCs) were prepared from Iranian Blood Transfusion Organization in Tehran. Sampling of the bags and isolation of PMP was carried out during storage. Besides, the respiratory burst of monocytes was measured by flow cytometry technique before and after treatment with PMP using di-hydrorhodamine-123 (DHR-123).
Results: Monocyte cells derived from human peripheral blood were activated after exposure to platelet microparticles and intracellular respiratory burst was significantly increased (P-value = 0.01). These findings suggest that PMPs have a dose-dependent effect on monocytes and they are more effective at the concentration of 500 micrograms per milliliter.
Conclusion: This study showed the potential of platelet microparticles in the stimulation and activation of human peripheral blood monocytes. |
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| Keywords: Blood Platelets, Cell-Derived Microparticles, Monocytes, Respiratory Burst |
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Full-Text [PDF 624 kb]
(1103 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2015/10/25 | Accepted: 2016/04/11 | Published: 2016/06/23
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