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Effects of eight weeks aerobic training on levels of amyloid β42, neprilysin and γ- secretase in the hippocampus of male rat Alzheimer’s model by homocysteine injection
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A. li Yaghoubi   , Marziyeh Saghebjoo , Z. ia Fallah-Mohammadi , Mehdi Hedayati , Akbar Hajizadeh Moghaddam |
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Abstract: (5511 Views) |
| Introduction: The aim of present study was to investigate the effects of eight weeks aerobic training on amyloid β42 (Aβ1-42), neprilysin and γ-secretase levels in the hippocampus of male rat Alzheimer's model by homocysteine injection.
Materials and Methods: For this purpose, 50 male Wistar rats (12 weeks old) were divided into five groups, including: Alzheimer’s control, Alzheimer's training, healthy control, healthy training and sham. To induce Alzheimer's, homocysteine, with a concentration of 0.6M, was infused into the rat cerebroventriculum. To assess the memory impairment induced by homocysteine, the shuttle box test was used. Training groups, exercised using treadmill with 27m/min at 0◦ slope (75-80% VO2max, 60min/day, 5 days/week). Amyloid β42, neprilysin and γ- secretase levels were measured using rat ELISA kit. Results: The results showed that Aβ1-42 levels in the hippocampus of Alzheimer's control group was significantly higher than healthy control and healthy training group (P=0.001). The level of γ- secretase in Alzheimer's control group was significantly higher than healthy control, healthy training and sham groups (P=0.001). Likewise, levels of this factor in Alzheimer's training group were significantly lower than Alzheimer's control group (P=0.001). No significant differences were found between neprilysin levels in different groups (P=0.07). Mean level of Aβ1-42 showed a significant positive correlation with γ- secretase level (r = 0.54 P = 0.001).
Conclusion: Based on these results, it s likely that continuous physical training can markedly reduce Aβ level in the hippocampus through reducing the level of γ- secretase and would be warrant to be considered as a complementary therapy in Alzheimer's disease |
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| Keywords: Exercise Therapy, Alzheimer Disease, Neprilysin, Homocysteine |
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Full-Text [PDF 894 kb]
(1557 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2015/07/22 | Accepted: 2016/02/10 | Published: 2016/06/23
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