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Ethanol- induced chromosomal abnormalities in human fibroblast cells
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Pooneh Aminigram   , Majid Rezaei Tavirani , Mona Zamanian Azodi , Bahador Bagheri , Reza Nasr , Mohammad reza Akbari Eidgahi |
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Abstract: (6297 Views) |
| Introduction: ethanol consumption may impose cytotoxic
effects on the human body, by producing toxic metabolites through different
pathways. Furthermore, some studies have also linked alcohol consumption to
various types of diseases. Therefore, this in vitro study is aimed to assess
the ethanol toxic effects on the human fibroblastic chromosome structure. Materials and Methods: G banding staining method was used to determine the
karyotype of chromosomal abnormalities of cultured fibroblast cells. Karyogram
of ethanol-treated cells after 48 hours of incubation with 54 and 108 mmol of
ethanol were assessed against the untreated cultured cells. Results: the
comparison between two karyogram models confirmed that lower ethanol
concentration (54mmol) caused breakage in chromosome type C and number 1, while
with higher concentration (108mmol) breakage was observed in chromosome 9,
chromatids as well as endoreplication in some other cells. Conclusion: this study indicates that specific
concentrations of ethanol can cause vast alterations in chromosomal structure.
Therefore, in general, more care is suggested in consuming this substance. In
addition, to confirm the cytotoxic alterations in chromosomal structure in
relation to alcohol consumption, using other cell lines and /or in vivo
studies, more investigations are warrant |
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| Keywords: Ethanol, Human fibroblast cell, Chromosome abnormalities, Karyotype |
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Full-Text [PDF 1482 kb]
(1502 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2014/04/13 | Accepted: 2014/10/22 | Published: 2014/12/16
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