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Evaluation of biochemical and molecular techniques in the diagnosis of urinary isolates of Enterobacter cloacae
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Majid Akbari   , Bita Bakhshi , Shahin NajarPeerayeh |
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Abstract: (5036 Views) |
| Introduction: Enterobacter spp.
belongs to the family of Enterobacteriaceae and has a variety of
phenotypic and genotypic profiles. Within Enterobacter cloacae complex,
six genetically related and phenotypically similar species have been merged and
strains belonging to these species are encountered among the most common
strains able to cause severe opportunistic infections in hospitalized patients. Sequence analyzing a
segment of hsp60 gene belong to this complex have demonstrated 13other
genetic clusters. Since this complex is rarely identified in Iran, therefore,
we aimed to evaluate the efficiency of phenotypic (commercial routine kit) and
molecular methods for detecting this complex in urinary isolates. Materials and Methods: In this study 74 Enterobacter cloacae urinary
isolates, collected from 6 large hospitals in Tehran, were characterized by
API20E kit and for final approval of diagnosis, hsp60 gene sequencing was performed. Results: Using
API20E kit for detecting Enterobacter cloacae in comparison to hsp60 sequencing, as gold standard for determining the species,
sub-species and genotypes in Enterobacter cloacae complex, have
demonstrated sensitivity and specialty about 88% and 60%, respectively in
bacteria detection. Discussion: Considering the importance and particular role of Enterobacter
cloacae complex in causing hospital-acquired and opportunist infections and
according to limited capability of API20E kit in providing precise analytic
data, performance of complimentary tests or molecular methods, such as hsp60 sequencing, with high accuracy and validity,
are highly suggested |
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| Keywords: Enterobacter cloacae, Chaperonin 60/genetics, Sensitivity and specificity, Cross Infection |
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Full-Text [PDF 1389 kb]
(1778 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2014/09/9 | Accepted: 2015/04/19 | Published: 2015/09/24
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