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Frequency and distribution of uterine macrophages on days 4.5 and 7 of pregnancy in NMRI mice
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Samira Gholizadeh   , Seyed Mohammad Moazzeni , Salehnia |
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Abstract: (4410 Views) |
| Introduction: Macrophages play a
crucial role in appropriate embryo implantation and maintenance of pregnancy.
This study was done to evaluate the frequency and distribution of uterine
(local changes) and spleen (systemic changes) macrophages on days 4.5 and 7 of NMRI mouse pregnancy
which are simultaneous with beginning of implantation and complete
establishment of embryo in the uterus. Materials and methods: The precise day of
pregnancy was determined through vaginal plug detection. Blood was collected
from pregnant mice on days 4.5 and 7 of gestation and serum
17- β estradiol and progesterone concentrations were measured using the ELISA
method. The frequency and localization of macrophages in different regions of
uterus and spleen were also investigated by immunohistochemistry staining using
anti F4/80 antibody. Results: The results of this study showed that
frequency of uterine macrophages has decreased on day 7 in comparison to day
4.5 of gestation. Macrophages are distributed throughout the whole uterus on
day 4.5 while their distribution was restricted to myometrium and stroma
beneath the circular muscles on day 7 of pregnancy. The frequency of spleen macrophages
as a representative of their systemic changes was not different in studied groups. Serum 17-β estradiol and progesterone concentrations increased
significantly on day 7 compared to day 4.5 of pregnancy. Conclusion: Considering
the changes in 17-β estradiol and progesterone concentrations and their
indirect effects on macrophages recruitment through regulation of M-CSF and
GM-CSF secretion by uterine stromal cells, the differences in frequency and
distribution of macrophages in different stages of pregnancy could be
explained. While the macrophages chemotactic factors and homing receptors and
their sources in spleen are different from uterus and don’t response to ovarian
hormones variations. Regarding the role
of macrophages in embryo implantation and regulation of feto-maternal immune
responses, it seems that the changes in their frequency and localization can be
helpful for a successful pregnancy |
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| Keywords: Macrophage, Uterus, Embryo implantation, Estradiol, Progesterone |
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Full-Text [PDF 1464 kb]
(1043 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2014/06/30 | Accepted: 2015/03/17 | Published: 2015/09/26
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