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Flowcytometric assessment of monocyte-derived dendritic cell efficiency for tumor cell lysate uptake in chronic lymphocytic leukemia patients
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P. کوخائی   , R. مهدیان , F. پاک , S. h. شهبازی , B. صدیقی مقدم |
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Abstract: (14765 Views) |
| Introduction: Dendritic cells (DCs) are the most potent antigen presenting cells in the
immune system. They are strongly involved in init iation of primary immune response ,
autoimmune disease, graft rejection, HIV infection and antitumor response. However, the
capability of DCs to activate tumor specific r cells in chronic lymphocytic leukemia (CLL) has
been reported, direct demonstration of soluble antigens of tumor lysate uptake had not been
shown. In this study we have shown uptake of antigen molecules by immature DCs using Flow
cytometric techniques.
Material and Methods: DCs of two CLL patients and two healthy donors have been
evaluated in this study. Peripheral blood monocytes have been purified using mouse anti-CDl4
coated magnetic beads and then' undergone culture process in the presence of rhGM-CSF and
rhILA. Generated DCs have been assessed for their ability of uptake FITC-labeled proteins of
tumoural B cells lysate by tlow cytometry.
Results: Fluorescence intensity in DCs pulsed with FITC-labeled tumor lysat e , after using
surface tluorescence quencher (trypan blue), were clearly more than unpulsed controls. This was
true for both CLL patients DCs and healthy donors DCs .
Conclusion: Immature DCs are capable to uptake soluble protein molecules of tumor lysate
prepared from B cells of CLL patients. |
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| Keywords: Flowcytornetry, Dendritic cell, Chronic Lymphocytic Leukemia, Lysate, Tumor cell lysate |
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Full-Text [PDF 1280 kb]
(2348 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2008/11/29 | Published: 2003/02/15
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