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Prevalence of genes encoding aminoglycoside resistant in methicillin-sensitive Staphylococcus aurous and coagulase-negative staphylococci isolated from hospital infectious
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Nooshin Abdal   , ٍEhsanolah Ghaznavirad , Adel Hamidi , Davood Hosseini |
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Abstract: (5933 Views) |
| Introduction: Staphylococci are the most common causes of
nosocomial infections are considered. Aminoglycosides are often used in
combination with B-lactamas and glycopeptides for the treatment of endocarditis
and bacteremia caused by Staphylococci. The main mechanism of aminoglycoside
resistance in staphylococci is drug inactivation by cellular aminoglycoside
modifying enzymes. Materials and Methods: 50 isolates of methicillin-sensitive
Staphylococcus aureus and 50 isolates coagulase-negative staphylococci, were
collected from various clinical specimens and were identified by standard
biochemical tests. The antibiotic susceptibility pattern of isolates using the
disk diffusion method and Etest for determining aminoglycoside antibiotics and
the frequency of gene aac(6')-Ie-aph(2"), aph(3')-IIIa and ant(4')-Ia was
determined using PCR. Results: 26% of the samples showed resistance to at
least one antibiotics.Genes aac(6')-Ie/aph(2") and aph(3')-IIIa were most
abundant genes, respectively. Approximately 14% of these genes were two samples
simultaneously, but in no instance a gene ant(4')-Ia were found. Conclusion: High prevalence of genes aac(6')-Ie-aph(2")
and aph(3')-IIIa resistance genes among isloate were found. Proper antibiotic
can be prescribed to prevent dissemination of resistant strains. Use phenotypic
and genotypic methods simultaneously give us full information of aminoglycoside
resistance |
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| Keywords: Coagulase, Staphylococcus aureus, Polymerase chain reaction, Aminoglycosides, Methicillin |
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Full-Text [PDF 1686 kb]
(1702 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2013/11/16 | Accepted: 2014/04/26 | Published: 2014/09/13
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