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Molecular identification of a 65 kDa mannoprotein encoded gene of Candida species by PCR
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Farahnaz Bineshian   , Mohamad Hossen Yadegari , Zohre Sharifi , Mohamad Reza Akbari Eidgahi , Reza Nasr |
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Abstract: (8725 Views) |
| Introduction: Invasive fungal infections represent a major public
health concern. In particular, systemic candidiasis remains an increasing
source of morbidity and mortality especially in immunocompromised patients such
as neutropenic patients undergoing antiblastic chemotherapy or bone marrow
transplants.
Early diagnosis is often difficult. Consequently, effective treatment is often
delayed. PCR has the potential to decrease the time required to diagnose fungal
infections and therefore reduce the mortality associated with disseminated
disease.The MP65 gene of Candida albicansis appropriate for detection and identification. The aimofthisstudy was
toidentifydifferentspecies of Candida (C. albicans, C.
glabrata, C. parapsilosis) withPCRtechnique. Materials and Methods: All 107 yeast
isolateswere identified on cornmeal agar supplemented with tween-80, germ tube
formation in serum, and assimilation of carbon sources in the API 20 C AUX
(Biomerieux, France). Then, all isolates were tesed by PCR using by different
species-specific PCR primers selected within the MP65 gene a recently cloned
gene encoding a mannoprotein adhesin. Results: A
hundred of clinical
isolates were detemined as C. albicans and 6 of the isolates detemined asC.
glabrata and 1 of the isolates detemined as C.
parapsilosis. The species-specific PCR
primers allowed differentiation of each of three Candida species by the
amplicon length produced.The primers amplified all
Candida species DNA tested (100% positivity) giving a band of the expected
length (475 bp for C. albicans, 361bp
forC. glabrata, 124bp forC.
parapsilosis). The results were agreed with all culture results for
Candida species detection. Conclusion: The results of this study showed that PCR method for
rapididentification ofCandida specieswith specific primers is reproducible, simple and specific |
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| Keywords: MP65 gene, Candida, Molecular diagnostics |
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Full-Text [PDF 335 kb]
(1849 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2013/02/24 | Accepted: 2013/11/5 | Published: 2014/03/10
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