Effects of anti-aflatoxin of essential oils of Cuminum cyminum, Ziziphora clinopodioiedes and Nigella sativa
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Mohammad Hassan Minooeian Haghighi , Alireza Khosravi |
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Abstract: (8238 Views) |
Introduction: Aflatoxins are a large group of mycotoxins, which are produced by certain
species of Aspergillus, including Aspergillus flavus, A. parasiticus and
A.numius. This group of fungal toxins is considered as a leader of all
mycotoxins. Therefore, many efforts have been performed for elimination or
neutralization of these compositions in foods and feeds. The aims of this study
were to survey the effects of anti-aflatoxin and anti-fungal essential oils of Cuminum
cyminum, Ziziphora clinopodioiedes and Nigella sativa growth in
Khorasan-e- Razavi province (Northeastern of Iran). Materials and Methods: In this experimental study, herbal substances which included Cuminum
cyminum, Ziziphora clinopodioiedes and Nigella sativa were selected
and collected on the basis of traditional medicine and their growing region.
Then, their essential oils (essences) were extracted by hydro-distillation
procedure. The concentration of each Aspergillus parasiticus aflatoxin
(B1, B2, G1, G2, and Total) was mesured three times by Reverse Phase-High
Performance Liquid Chromatography (RP-HPLC) technique in ng/ml (ppb). Also, the
final concentration of each aflatoxin was measured following the impact of Cuminum
cyminum, Ziziphora clinopodioiedes and Nigella sativa essences in
concentrations of 0.25, 0.25, and 1.5 mg/ml, respectively. Results: The inhibitory effect of above mentioned essential oils on producing of
different aflatoxins was the essences of Cuminum cyminum,Nigella sativa
and Ziziphora clinopodioiedes, respectively. Also, there was a
significant and positive correlation between fungus dry weight and amount of
total aflatoxin (P = 0.0005, r = 0.896). Conclusion: The findings of this study
showed that Cminum cyminum, Nigella sativa and Ziziphora
clinopodioiedes has an inhibitory effect on aflatoxin producing by
Aspergillus parasiticus. In addtion, this study confirmed the
traditional use of these herbs against microbial infections |
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Keywords: Essences, Cuminum cyminum, Ziziphora clinopodioiedes, Nigella sativa, Aspergillus parasiticus, RP-HPLC, Aflatoxins |
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Full-Text [PDF 450 kb]
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Type of Study: Research |
Subject:
General Received: 2013/02/1 | Accepted: 2013/11/26 | Published: 2014/03/11
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