Introduction: Several in vitro studies have shown the effect of external stimulus on acceleration of long bone fracture repair in experimental animals. The aim of this study was to determine feasibility and creation of appropriate conditions for proliferation of the bone cells, in flat and long bones of mice and rabbits . Materials and Methods: Mice and rabbits flat and long bones were cultured in 12 plates. At 14th day of culture, they reached an appropriate level of cell growth. Then specific staining methods were used to study the pattern of cell growth. Results: The results showed a significant differences (P