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HPLC measurment of phenylalanine in plasma, with application to patients with phenylketonuria
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A. R. بندگی   , M. دوستی |
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Abstract: (16927 Views) |
| Introduction. Diagnosis and treatment of phenylketonuria (PKU) requires
measurment of phenylalanine (Phe) in serum or plasma. The aim of this study was to
use a simple and rapid method for measurment of Phe.
Materilas and Methods. Phe is determined by isocratic high performance liquid
chromatography (HPLC) method, with UV-VIS detection in 210 nm , octadecyl-silane (ODS) column and the mobile phase of aceton itrillwater. For this measurment 10 microliter plasma was used and complet analysis was done at less than 12 min from injection.
Results. Coeifficients of variation (% CV) for human plasma sample (Phe, 60-1200
mmol/L) ranged between 2.8% and 3.3%. The method has wide linear range (10-3000
mmol/L).
Conclusion. In this method preparation of sample is simple and rapid. This method
has an high efficiency for measurment of Phe concentration in the normal and PKU
subjects. |
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| Keywords: Phenylketonuria, Phenylalanine, Measurment, Plasma, High Performance Liquid Chromatography |
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Full-Text [PDF 868 kb]
(2550 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2009/02/2 | Published: 1999/11/15
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