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In vitro anti-bacterial effect of nano-polyamidoamine-G5 dendrimer
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Soudabeh Alizadeh Matboo   , Shahram Nazari , Leila Mohammadi , Susan Bagheri , Fakhraddin Akbari Dourbash , Ahmadreza Yari , Seyyed Ahmad Mokhtari , A. li Niapour , Seyyed Mohsen Mohseni |
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Abstract: (4217 Views) |
| Introduction: Progress in nanotechnology in the past decayed has created various opportunities for evaluation of biological effects such as anti-bacterial effects of nanoparticles. This study was aimed to examine synthesis and the antibacterial effect of Nano-Polyamidoamine-G5 (NPAMAM-G5) dendrimer on Klebsiella Pneumoniae, Pseudomonas Aeruginosa, Shigella Dysenteriae and Bacillus Subtilis.
Materials and Methods: NPAMAM-G5 dendrimers was synthesized by Tomalia’s divergent growth approach. The antibacterial effects of NPAMAM-G5 dendrimer were studied by disc diffusion and micro-dilution method. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against gram-positive and gram-negative bacteria were determined according to Clinical and Laboratory Standards Institute (CLSI) guideline. Transmission electron microscopy (TEM) was used to analyze morphology and size of NPAMAM-G5.
Results: Zone of inhibition in concentration 25μg/ml of NPAMAM-G5 dendrimers for Klebsiella Pneumoniae, Pseudomonas Aeruginosa, Shigella Dysenteriae and Bacillus Subtilis were 27, 13, 30 and 18 mm, respectively. There was a significant difference regarding the zone of inhibition between gram-negative and gram-positive bacteria (p |
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| Keywords: Gram Positive Bacteria, Gram Negative Bacteria, Dendrimers, Polyamines, Microbial Sensitivity Tests |
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Full-Text [PDF 1995 kb]
(972 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2016/09/10 | Accepted: 2018/08/1 | Published: 2019/01/7
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