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Assesment, isolation and identification of cyclosporin A by gel chromatography, fungicidal activity and spectroscopy
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M. غروی   , N. خدائی , R. بحری نجفی |
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Abstract: (16499 Views) |
| Introduction:T .inflatum (To lypocladium inflaturn) is a pr imitive fu ngi tha t rep ro ducts via
co nidiu ms. Spors grow up on malt extract cultu re at 6-32nC at wet o r dry medium. Of co urse it is
found o f so me othe rs culture wh ich are co nt ain a minoaci ds , min e ra l o r orga n ic ma tt er.
Cyclosporin A (Cy A) is the me tabolit e of l.inlla tum. Cy A is an immu nos up press ive d rug. Th e
aim o r t hi s s t udy was to promo te pr oductio n o r Cy A by mut at ion via ult raviol et ra dia tio n
(UVR) and to find out a suitable med ium for auxotrop hs.
Material and Methods: Tolypocladium inflatum (OSM 915), malt extrac t and sabo urd 4%
dextrose aga r, Bactop e pt amine , casein hydrolysate and yeast extract dehydrated. The fungi was
cultivate d for 10-20 days duration o n a medium contains 2% malt extract, 4% yeas t extrac t and
20% aga r at pH 5.7 . Ex trac tio n was car ried o ut by gel chromatograp hy. For mu ta t ion o f t he
fungi. UVR was used. and for identification of Cy A fungicidal, ultraviolet. visible and infra -red
spec tros co py were methods of choice.
Results: Whi te ve lvey co lo nie s we re co llected from the medium of agar and malt ext ra ct.
Samples and fra ctions of gel chromatography extraction showed an tifungal act ivity o n aspergillus
niger. UItr avi ol et vis ible spec t rosco py gave Amax si mila r to tha t o f s t a nda rcd a nd infr a- re dspec tra was similar to the standa red spec tra.
Conclusion: Auxotrophic str ains we re cultivated on synthe tic and raw mediums. Finally the
high produ ct ion of Cy A was produced of sugar fact roy and hogwas h of corn oil press ing tac troy,
at a dark place. |
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| Keywords: Cyclosporin, Fermentation, Chromatography, Mutation, Fungicidal activity |
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Full-Text [PDF 2505 kb]
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Type of Study: Research |
Subject:
General
Received: 2008/12/23 | Published: 2002/08/15
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