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Study of colorectal polyps using 1H Nuclear Magnetic Resonance spectroscopy
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Sedigheh Sadeghi   , Ayda Iravani , Mohammad Arjmand , Farideh Vahabi , Syedmahmood Eshaghhoseini , Akbar Oghalai , Fatemeh Mirkhani , Zahra Zamani |
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Abstract: (4707 Views) |
| Introduction: The patients with polyps and ulcerative colitis diagnosis are more susceptible to colorectal cancer. So far, the diagnosis of colorectal diseases has been dependent on invasive procedures, such as sigmoidoscopy and colonoscopy. However, some recent research has been initiated for early diagnosis of colon cancer by using1H nuclear magnetic resonance (1H NMR) spectroscopy and chemometrics methods. In this study, spectrum results of patients and samples of normal subjects were compared.
Materials and Methods: Participants who referred for colonoscopy (n=40) filled a consent form.They had received liquid diets for last 72 hours. Blood samples were collected in heparinized tubes. Samples were collected from patients who were diagnosed with polyps and also normal subjects. The separated plasma samples were sent for 1HNMR spectroscopy using CPMG Spin-echo methods. The samples were analyzed using ProMetab software, with performance of Principle Component Analysis. The different metabolites were identified by their chemical shifts.
Results: There were 1624 metabolites in each spectrum. Effective metabolites were detected using Human Metabolome Data Base and effective metabolic cycle were determined using metaboanalyst Data Base.
Conclusion: These findings indicated that the metabolism of amino acid tRNA synthase, histidine, cyanoamine and thiamine are the main differentiating metabolic cycles involved in the production of colorectal polyp. |
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| Keywords: Colorectal Polyp, Colorectal Cancer, Spectroscopy, Chemometrics |
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Full-Text [PDF 581 kb]
(1143 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2014/06/14 | Accepted: 2016/01/11 | Published: 2016/06/23
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