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Investigating the possibility of recombination the predicted antigenic fragment of Streptococcus Pyogen hyaluronidase by bioinformatics softwares
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Safieh Soufian   , Shabnam Sadoogh Abbasian , Ali Reza Japoni Nejad , Hamid Abtahi |
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Abstract: (4650 Views) |
| Introduction: One of the main difficulties facing the production of
recombinan Streptococcus pyrogenesisa hyaluronidase enzyme is its high molecular
weight. The aim of this study was to predict the antigenic regions of
hyaluronidase protein, the structure of fragment and evaluating the possibility
of cloning the fragment in pTZ57R/T vector by the aid of bioinformatic’s software. Materials and Methods: Multiple hyaluronidase amino acid sequences were aligned,
using Clustal W method and MegAlign software, followed by BLASTP analysis.
Antigenic regions and important epitopes in consensus sequences were determined
by using online softwares ABCpred, BcePred and Emboss. The tertiary structure
of determined antigenic regions was predicted by using TASERI and specified
model was evaluated by using Swiss-PDB Viewer Software. Finally, intended
fragment was inserted in pTZ57R/T vector. Results: The
consensus sequence had 868 amino acids, which the residues 279 to 773 were
selected as prominent antigenic region in order to shorten the sequence by using
online software. Finally, the result was shown as the proper recombination of
antigenic region of hyaluronidase gene with pTZ57R/T. Conclusion: The utilization of bio informatics tools to predict or determine the
prominent antigenepitopic fragments, facilitated the recombinant process due to
using the shorter fragments and generating more effective and specific immune
response by the enzyme protein |
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| Keywords: Hyaluronidase enzyme, Antigenic regions, Cloning |
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Full-Text [PDF 790 kb]
(1333 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2014/02/1 | Accepted: 2015/04/29 | Published: 2015/09/24
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