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:: Volume 15, Issue 3 (Spring 2014, 15 (3). 275-404 2014) ::
koomesh 2014, 15(3): 359-364 Back to browse issues page
Survey on CCR5-Δ32 mutation in healthy individuals and patients with chronic hepatitis B referred to the clinical laboratory of Iranian Blood Transfusion Organization
Sajjad Jalali , Zohreh Sharifi * , Mohammad Hossein Sanati , Abolhassan Shahzadeh Fazeli
Abstract:   (4925 Views)
 

Introduction:CCR5 is one of the most important chemokine receptor involved in the recruitment of specific anti-viral immune cells to the liver. CCR5-Δ32 is a functionally null allele of CCR5 gene with a 32-bp deletion. Several studies have reported that this mutation may be associated with recovery from acute hepatitis B or persistence of HBV infection. The main purpose of this study was to compare the frequency of the Δ32 mutation within the CCR5 gene in a group of chronic HBV infected patients with healthy individuals in Iran.

 

Materials and Methods:A total of 200 blood samples including 100 healthy individuals and 100 HBsAg positive patients were randomly selected. The samples were tested for HBsAg by ELISA and HBV-DNA by PCR method. Genomic DNA was extracted by salting out method. CCR5-Δ32 mutation was genotyped by PCR with specific primers.

 

Results:The results of this study showed that none of the subjects in the control and patient groups had a 32-bp deletion in the CCR5 gene. CCR5-Δ32 was not a prevalent mutation in control group and HBV infected patients in Iran.

 Conclusion:No significant difference was observed in genotypes frequency of CCR5-Δ32 between control group and HBV infected patients, therefore it seems that this mutation is not associated with persistence of HBV infection in Iranian patients
Keywords: CCR5, Chronic hepatitis B, Mutation
Full-Text [PDF 307 kb]   (772 Downloads)    
Type of Study: Research | Subject: General
Received: 2013/08/20 | Accepted: 2013/11/20 | Published: 2014/03/10
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Jalali S, Sharifi Z, Sanati M H, Shahzadeh Fazeli A. Survey on CCR5-Δ32 mutation in healthy individuals and patients with chronic hepatitis B referred to the clinical laboratory of Iranian Blood Transfusion Organization. koomesh. 2014; 15 (3) :359-364
URL: http://koomeshjournal.semums.ac.ir/article-1-2182-en.html


Volume 15, Issue 3 (Spring 2014, 15 (3). 275-404 2014) Back to browse issues page
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