اکثر مکاتبات کومش از طریق ایمیل سایت می باشد. لطفا Spam ایمیل خود را نیز چک نمایید.
   [Home ] [Archive]   [ فارسی ]  
:: ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
Subscription::
Contact us::
Site Facilities::
Webmail::
Editorial Board::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
:: Volume 15, Issue 1 (پائيز 1392، پياپی 49 1392) ::
Koomesh 1392, 15(1): 11-16 Back to browse issues page
Effects of in vitro expansion of CD133+ hematopoietic stem cells isolated from umbilical cord blood in expression of microRNAs involved haematopoiesis
Saeid Shahrabi , Saeid Kaviani , Masoud Soleimani , Ali akbar Pour fatollah , Zahra Zonoubi
Abstract:   (8110 Views)
 Introduction: Hematopoiesis is regulated by different microRNAs (miRNA). These small regulatory RNAs control hematopoiesis at different stages with changing in the expression of genes at post-transcriptional levels. Treatment of various diseases is increasing with using CD133+ hematopoietic cells isolated and in vitro expanded from the umbilical cord blood. This study was performed to show alteration of microRNAs levels involved in hematopoiesis in CD133+ hematopoietic cells isolated and invitro expanded from the umbilical cord blood. Materials and Methods: Umbilical cord blood CD133+ hematopoietic stem cells were isolated by MACS and then the cells confirmed and counted by using flowcytometery and finally were divided into two groups .In the first group RNA was extracted from the cells and the cells in the second group were cultured invitro for 12 days and then these cells were used to assess micoRNAs expression using qPCR real time. Results: The results showed that from 23 microRNAs, expression of 11 microRNAs was the same in two groups whereas expression of 7 and 5 microRNAs were respectively increased and decreased following in vitro culture. Conclusion: Based on our results, in vitro expansion of the hematopoietic stem cells results in increased microRNAs levels which are responsible for differentiation and decreased microRNAs levels which are responsible for self- renewal. 
Keywords: Hematopoiesis, MicroRNAs, Hematopoietic Stem Cells+CD133, Fetal Blood
Full-Text [PDF 296 kb]   (1742 Downloads)    
Type of Study: Research | Subject: General
Received: 2012/11/26 | Accepted: 2013/09/8 | Published: 2013/09/10
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

shahrabi S, kaviani S, Soleimani M, Pour fatollah A A, Zonoubi Z. Effects of in vitro expansion of CD133+ hematopoietic stem cells isolated from umbilical cord blood in expression of microRNAs involved haematopoiesis. Koomesh 1392; 15 (1) :11-16
URL: http://koomeshjournal.semums.ac.ir/article-1-1919-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 15, Issue 1 (پائيز 1392، پياپی 49 1392) Back to browse issues page
کومش Koomesh
Persian site map - English site map - Created in 0.05 seconds with 38 queries by YEKTAWEB 4645