Introduction: Hematopoiesis is regulated by different microRNAs (miRNA). These small
regulatory RNAs control hematopoiesis at different stages with changing in the
expression of genes at post-transcriptional levels. Treatment of various diseases
is increasing with using CD133+
hematopoietic cells isolated and in vitro expanded from the umbilical cord
blood. This study was performed to show alteration of microRNAs levels involved
in hematopoiesis in CD133+ hematopoietic cells isolated and invitro expanded
from the umbilical cord blood. Materials and Methods: Umbilical cord blood CD133+ hematopoietic stem cells were isolated by
MACS and then the cells confirmed and counted by using flowcytometery and
finally were divided into two groups .In the first group RNA was extracted from
the cells and the cells in the second group were cultured invitro for 12 days
and then these cells were used to assess micoRNAs expression using qPCR real time. Results: The results showed that from 23 microRNAs, expression of 11 microRNAs was
the same in two groups whereas expression of 7 and 5 microRNAs were
respectively increased and decreased following in vitro culture. Conclusion: Based on our results, in vitro expansion of the hematopoietic stem cells
results in increased microRNAs levels which are responsible for differentiation
and decreased microRNAs levels which are responsible for self- renewal.
shahrabi S, kaviani S, Soleimani M, Pour fatollah A A, Zonoubi Z. Effects of in vitro expansion of CD133+ hematopoietic stem cells isolated from umbilical cord blood in expression of microRNAs involved haematopoiesis. Koomesh 1392; 15 (1) :11-16 URL: http://koomeshjournal.semums.ac.ir/article-1-1919-en.html