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Cloning, expression and culture optimization of gene encoding Aspergillus niger NRRL3135 phytase in Hansenula polymorpha host
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Mohammadtaghy Borjian Borujeni   , Omid Ranaei Siadat , Javad Harati , Farnaz Nikzad jamnani , Shirin Yousefian , Sohrab Moradi |
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Abstract: (11580 Views) |
| Introduction: Phytase is able to change phytic acid into
the myo-inositiol and inorganic phosphate, so it has been used as a cereal food
additive in monogastric animal’s food.
The aim of this study was to eamine cloning, expression and culture
optimization of gene encoding Aspergillus niger NRRL3135 phytase in Hansenula
polymorpha host. Materials and Methods: In this study, in order to achieve high level production of protein, the
sSequence of protein encoding fangal Aspergillus niger NRRL3135 phytase
was designed according to the expression cod on usage of Hansenula polymorpha
and transformation was done. The enzyme specific activity in different cultures
was survived. Results: The results in flask culture showed that the recombinant phytase
production was 1185 U/L with 34.84 U/mg specific activities in YNB-Methanol
culture. Conclusion: Results indicate YP-methanol
culture is the efficient culture to produce Aspergillus niger NRRL3135
phytase in Hansenula polymorpha as a host on industrial scale |
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| Keywords: Phytsase, Culture optimization, Hansenula polymorpha |
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Full-Text [PDF 318 kb]
(2695 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2012/10/21 | Accepted: 2013/09/8 | Published: 2013/09/10
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