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Detection and cloning of desulfurization operon sox(dszA&B) in rhodococcus FMF native bacterium
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S. اکبرزادهشعرباف   , J. راهب |
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Abstract: (13034 Views) |
| Introduction: Native Rhodococcus FMF bacterium was selected among several isolated Iranian bacterium. Primary studies have shown that the bacterium use dibenzothiophene as a sulfur source. Since desulfurization is taken by a biochemical pathway, the present project was designed to detect, clone and sequence of desulfurization genes of the bacterium.
Materials and Methods: Desulfurization operon was isolated from Rhodococcus erythropolis IGTS8 and was used as a probe for detection of desulfurization 4S pathway genes by southern blotting technique. Specific primers were designed and the dszA and B genes were amplified by polymerase chain reaction (PCR). PCR products were recovered from agarose gel and were cloned into the PTZ57R vector.
Results: In this study, recombinant PTZAB57R vector was constructed by the insertion of dszA and B genes into the PTZ57R vector. The clones were confirmed by restriction digestion analysis. The PTZAB57R was extracted by large-scale method and was sequenced (MWG DNA Biotech).
Conclusion: Comparison of sequences of dszA and B genes from native Rhodococcus FMF bacterium with Rhodococcus sp. IGTS8 bacterium were shown complete identity between them, which show that desulfurization pathway is conserved in this bacterium. |
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| Keywords: Rhodococcus sp., Rhodococcus erythropolis IGTS8, Biodesulfurization, Dibenzothiophene |
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Full-Text [PDF 759 kb]
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Type of Study: Applicable |
Subject:
General
Received: 2008/09/14 | Published: 2004/08/15
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